WebApr 24, 2024 · Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different … Web寻找 Abcam 抗体及试剂 - Abcam ... Human Cytokine Antibody Array (160 Targets) - Quantitative. Reactivity: Human. Sample type: Serum, Plasma, Cell Lysate, Cell culture media, Tissue Lysate. Human Cytokine Antibody Array (200 Targets) - Quantitative (ab197428) Description:
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WebApr 13, 2024 · For rabbit-raised antibodies, a biotin-free immunoenzymatic detection kit was used (Expose Rabbit Specific HRP/DAB Detection Kit, ab80437 Abcam, UK), whereas for mouse-raised antibodies, a double-stain IHC kit (M&R on human tissue, DAB & AP/Red, ab210059, Abcam, UK) was used. WebA visual comparison between the four arrays performed by two independent investigators and by ImageJ software (National Institutes of Health) revealed that five cytokines were especially affected by the treatment: tumor necrosis factor-α (TNF-α), interleukin-1α (IL-1α), T cell activation gene-3 (TCA-3), stromal cell-derived factor-1 (SDF-1 ... northampton wine + dine
Human IL-1 Family Cytokine Antibody Array (11 Targets)
Webab197443 can be used for quantitative measurement of 40 Human cytokines. Suitable for serum, plasma, cell culture media, other body fluids, cell and tissue lysates. Human Cytokine Antibody Array F (40 Targets) - Quantitative (ab197443) Abcam WebApr 13, 2024 · ImageJ-protein array was used to quantify spot density; then, the background signal was subtracted from each value. Finally, we compared the spot density on different arrays to evaluate the relative changes in cytokine/chemokine levels between sham and day 3 ICH groups. A total of 8 mice were used in this experiment (n = 4 sham … WebCytokine Array. A total of 100,000 cells were seeded on 35 mm plates and exposed to 300 µg/mL HA working solution in 2 mL media. The supernatant was collected on day 3 and subjected to Human Inflammation Antibody Array (Abcam; USA) overnight. The kit protocol was followed for the subsequent steps. Transmission Electron Microscopy (TEM) how to replace a crossbow string