Plvx-shrna2-puro
WebpLVX-shRNA2 Vector Information . PT4052-5 Catalog No. 632179. pLVX-shRNA2 Vector Map and Multiple Cloning Site (MCS). Description. pLVX-shRNA2 is an HIV-1-based, … WebpLVX-shRNA2 Vector Information . PT4052-5 Catalog No. 632179. pLVX-shRNA2 Vector Map and Multiple Cloning Site (MCS). Description. pLVX-shRNA2 is an HIV-1-based, …
Plvx-shrna2-puro
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WebThe pLVX-shRNA2 lentiviral transfer plasmid was associated with the highest lentiviral titer, while both pLVX-shRNA2 and psi-LVRU6GP plasmids were associated with efficient RNA … WebBiowit Technologies lentiviral shrna vector plasmid plvx shrna2 puro amp Lentiviral Shrna Vector Plasmid Plvx Shrna2 Puro Amp, supplied by Biowit Technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations.
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WebpSuper pSuper retro GFP-neo pSupeior puro pBabe puro pCMV-Gag-pol pCMV-VSV-G pCL Ampho pSuper retro puro pLVX-shRNA1 pLVX-shRNA2 pSicoR pSicoR pGK puro pLentilox 3.7 pLKO.1 puro PLKO.1 pLKO.3G pTet-PLKO-puro pPRIME-TET-GFP-FF3 pPRIME-TREX-GFP-FF3 pSIH1-H1-CoGFP pGIPZ empty pGIPZ control pTRIPZ empty pTRIPZ control pLVX … WebJul 30, 2024 · Subsequently, shRNAs were ligated to the pLVX-shRNA2-puro plasmid using a T4 DNA ligase, synthesizing the pLVX-shPOU4F2-1-puro, pLVX-shPOU4F2-2-puro, and pLVX-shNC-puro interference plasmids. These plasmids were transformed into DH5α competent cells, and consecutively their correct sequence was verified by restriction …
WebAug 14, 2015 · zt1248 pLVX-aMHC Puro addgene 慢病毒过表达载体. zt1249 pLVX-cas9 addgene 慢病毒过表达载体. zt1250 pLVX-cGFPI addgene 慢病毒过表达载体. zt1251 pLVX-cGFPII addgene 慢病毒过表达载体. zt1252 pLVX-CMV addgene 慢病毒过表达载体. zt1253 pLVX-EFGL addgene 慢病毒过表达载体
WebpLVX-shRNA2-puro vector contained scramble shRNA or empty pLVX-puro vector transfection were used as negative controls. The primers for plasmid construction were listed in Table S1. The transfection eciency was determined by RT-PCR. CCK‑8 Assay A Cell Counting Kit-8 (CCK-8) assay kit (96,992; Sigma-Aldrich) was used to determine cell viability. great restaurants in huntington beachWebpLVX-shRNA2-Puro: 质粒类型: RNAi载体: 高拷贝/低拷贝: 高拷贝: 克隆方法: 限制性内切酶，多克隆位点: 启动子: U6: 载体大小: 8998 bp: 5' 测序引物及序列:--3' 测序引物及序列:--载 … great restaurants in irvineWebWe synthesized the GTF2IRD2P1 sequence and subcloned it into plasmids pLVX-IRES-ZsGreen1 and pLVX-shRNA2-puro to obtain the overexpression and low expression group plasmids, respectively. Each plasmid was then packaged with lentivirus. Transfection was performed with the Liposome 2000 reagent (ThermoFisher Scientific, Waltham, MA, USA) … great restaurants in houstonWebpLVX-shRNA2 is an HIV-1-based, lentiviral expression vector designed to express a small hairpin RNA (shRNA) for RNA interference (RNAi) studies. Expression of your shRNA is … great restaurants in kingston jamaicaWebJan 20, 2024 · Results:The interference sequence of 3β-HSD gene inserted into lentivirus vector PLVX-shRNA2-puro is consistent with the designed sequence. 3β-HSD gene … great restaurants in indianapolisWeb首先将靶向沉默TOE1基因的shRNA序列插入慢病毒载体pLVX-shRNA2-Puro，构建敲低TOE1基因的慢病毒载体pLVX-shTOE1。然后通过PCR扩增TOE1基因序列克隆至慢病毒载体pCDH-CMV-MCS-EF1-Puro，构建TOE1基因过表达的慢病毒载体pCDH-TOE1。同时构建pEGFP-TOE1真核表达载体，转染人胃癌细胞SGC ... great restaurants in key west flWebNov 29, 2024 · Then, SH-SY5Y cells were collected for further experiments. Cells with agomir-NC, antagomir-NC, pLVX-shRNA2-puro vector contained scramble shRNA or empty pLVX-puro vector transfection were used as negative controls. The primers for plasmid construction were listed in Table S1. The transfection efficiency was determined by RT … great restaurants in knoxville tn